ABSTRACT

During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues.

Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics.

Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.

part I|6 pages

Overview

chapter 1|6 pages

Overview

part II|207 pages

The Techniques for Gene Transfer

section Section A|38 pages

Gene Transfer into Mammal Embryos

section Section B|15 pages

In Vitro Mammal Embryo Manipulation

section Section D|28 pages

Gene Transfer into Fish and Lower Vertebrates

section Section F|31 pages

The use of ES Cells for Gene Transfer and Gene Targeting

section Section G|25 pages

The Direct Gene Transfer into Somatic Cells of Animals

part III|129 pages

The Fate of Microinjected DNA into Mammal Embryos

section Section B|93 pages

The Vectors for Transgenesis

chapter 42|6 pages

Transgene Rescue

part IV|180 pages

The Use of Transgenic Animals

section Section B|58 pages

The Generation of Animal Models for Biomedical and Pharmaceutical Studies

section Section D|10 pages

The Modification of Milk Composition

part V|50 pages

The New Problems Generated by Transgenic Animals