ABSTRACT
The identification of disease-specific proteins by proteome analysis has been tried to date for malignant tumors such as lung cancer and diffuse lung diseases, including idiopathic pulmonary fibrosis (IPF), sarcoidosis, and pulmonary alveolar proteinosis. Combinations of two-dimensional polyacrylamide gel electrophoresis (2DPAGE), high-performance liquid chromatography (HPLC), and surface-enhanced laser desorption/ionization (SELDI) with mass spectrometry (MS) (2D-PAGE/MS, LC/MS/MS, and SELDI-TOF-MS, respectively) have been employed for analysis. This chapter outlines approaches to identifying proteins specific to these pulmonary diseases.
