ABSTRACT
Synthetic polymer matrices that include pre-designed recognition
sites provide interesting interfaces for sensor devices [1]. Polymers
that include receptor molecular units such as crown-ethers [2],
cyclodextrins (CDs) [3], and other recognition modules [4] have
been employed as active-sensing interfaces. Biosensors are
‰‡‡”ƒŽŽ›Š‹‰ŠŽ›•’‡…‹ϐ‹…ƒ•ƒ”‡•—Ž–‘ˆ•’‡…‹ϐ‹…”‡…‘‰‹–‹‘„‡–™‡‡ …‘’Ž‡‡–ƒ”› „‹‘Ž‘‰‹…ƒŽ •–”—…–—”‡•Ǥ ‘™‡˜‡”ǡ –Š‡› ‘ˆ–‡ •—ơ‡” from limited stability, and are applicable to a narrow group of
„‹‘Ž‘‰‹…ƒŽ‘Ž‡…—Ž‡•Ǥ”–‹ϐ‹…‹ƒŽ…Š‡‹…ƒŽ•‡•‘”•ƒ”‡‡š’‡…–‡†–‘‰‹˜‡ •—„•–ƒ–‹ƒŽŽ›Š‹‰Š‡”•–ƒ„‹Ž‹–‹‡•ƒ†™‘—Ž†„‡‡ơ‡…–‹˜‡ˆ‘”•‡•‹‰‘ˆ
larger varieties of substances including biological substrates, though
•’‡…‹ϐ‹…‹–›ƒ†•‡Ž‡…–‹˜‹–›ˆ‡ƒ–—”‡•ƒ›„‡Ž‡••–Šƒ•ƒ–‹•ˆƒ…–‘”›Ǥ Molecular imprinting is a representative technique for the
ˆƒ„”‹…ƒ–‹‘ ‘ˆ ƒ”–‹ϐ‹…‹ƒŽ ”‡…‡’–‘”•ǡ™Š‹…Š ƒ”‡ ƒ„Ž‡ –‘ „‡ ƒ’’Ž‹‡† –‘ sensing, catalysis and separation materials. The advantages of
molecularly imprinted materials over biological receptors include
their mechanical and chemical stability, low preparation cost
ƒ†™‹†‡ ”ƒ‰‡‘ˆ‘’‡”ƒ–‹‰ …‘†‹–‹‘•Ǥ –Š‡ͳͻͲ•ǡ—Žơet al. proposed the synthesis of polymers that were substrate-selective
†—‡–‘•’ƒ–‹ƒŽϐ‹šƒ–‹‘‘ˆ•‡˜‡”ƒŽˆ—…–‹‘ƒŽ‰”‘—’•–Šƒ–‘…‘˜ƒŽ‡–Ž› interacted with the template molecules [5,6]. Similar studies
have been extensively carried out to the present time, and their
application to the separation of complicated organic compounds
and biologically active substances is most important [7-19]. In this
approach, a polymerizable functional monomer (i.e., methacrylic
ƒ…‹†ȋȌǡ˜‹›Ž’›”‹†‹‡Ȍ–Šƒ–™ƒ•…Š‘•‡ˆ‘”‹–•ƒˆϐ‹‹–›ˆ‘”‰‹˜‡ print molecules, was combined with a large excess of the cross-
linking agent (i.e., ethylene glycol dimethacrylate) and a free-radical
initiator to form a rigid polymer. After the template was removed,
complementary binding sites for the template were created. The
molecular recognition properties of these synthetically designed
binding sites are attractive and thus these materials have been
routinely applied to the development of chromatographic stationary
phases.