ABSTRACT
The microspheres used for solid-phase modification of proteins are usually the liquid chromatography media. Liquid chromato-graphy includes ion-exchange chromatography (IEC), size-exclusion chromatography (SEC), and affinity chromatography. These contain the liquid mobile phase and the stationary phase (13). Typically,
Tao Hu and Zhiguo Su
the stationary phase is mechanically stable and chemically inert under chromatographic conditions used. It is also desirable that the microspheres act as a support for chemical groups with desirable properties, such as hydrophobicity, ion exchange, or affinity ligands (14). Therefore, microspheres are most commonly used as the stationary phase (i.e., chromatography media) in chromatography of samples.
