ABSTRACT
When Terry Berger’s reference text on supercritical fluid chromatography (SFC) was published in 1995, mass spectral detection was mentioned only in four separate sentences, in the entire book [1]. Currently, a literature search yields about 150 references to supercritical fluid chromatography-mass spectrometry (SFCMS), where about one quarter of them show some application to the pharmaceutical industry, under the general categories of medicinal/ discovery chemistry [2,3], chiral separations [4], high-throughput screening [5-7], and biologics (lipids, membrane proteins, peptides) [8,9]. However, the most prevalent use of SFC-MS is its use for massdirected isolations and purifications [3,10].
