ABSTRACT

A knowledge of the practical aspects of clinical biochemistry is essential for medical students to understand the diagnostic and prognostic status of any ailment. This book addresses and compiles some of the invariably used protocols in biochemistry with the aim to enhance the understanding of the basics behind following the methodologies, including those for urine analysis; carbohydrate, protein, and lipid analyses; and diabetes and lipid profile. It discusses organ function tests, such as liver, renal, or pancreatic function tests, in addition to exploring pH and buffer, colorimeter, spectrophotometer, chromatography, electrophoresis, enzyme-linked adsorbent assay, radioimmunoassay, biosensor/glucometer, fructosamine, DNA isolation, mass spectrometry, flow cytometry, centrifugation, automation, cerebrospinal fluid examination, and oxalic acid test. The book also touches upon the basics of laboratory safety rules and laboratory glassware.

The book is helpful for students to understand the relationship between quality management and specimen collection and processing them for their biological functions. It is well-suited for academic courses, including undergraduate, graduate, postgraduate, and professional courses and covers the practical syllabus for MBBS, BDS, BSc MLT, BSc nursing, and MSc students. For undergraduate students in the disciplines of biochemistry, molecular biology, chemistry, and other related biological sciences, the book forms an integral part of the syllabus with its case studies, MCQs, biochemistry, OSPEs, etc. It also demonstrates the actual experiments for understanding the concepts better and is a must read for students to acquire skills to work with a clinical biochemistry laboratory.

chapter Chapter 1|16 pages

Lab Safety Rules

chapter Chapter 2|7 pages

Specimen Collection and Processing

chapter Chapter 3|6 pages

Quality Management in the Laboratory

chapter Chapter 4|29 pages

Urine Analysis

chapter Chapter 5|14 pages

Carbohydrates

chapter Chapter 6|15 pages

Blood Glucose

chapter Chapter 7|22 pages

Proteins

chapter Chapter 8|7 pages

Lipid Analysis

chapter Chapter 9|9 pages

Lipid Profile

chapter Chapter 10|17 pages

Liver Function Test

chapter Chapter 11|13 pages

Renal Function Tests

chapter Chapter 12|5 pages

Pancreatic Function Test

chapter Chapter 13|6 pages

Acids and Bases

chapter Chapter 14|6 pages

Colorimetry

chapter Chapter 15|16 pages

Chromatography

chapter Chapter 16|7 pages

Electrophoresis

chapter Chapter 17|10 pages

Enzyme-Linked Immunosorbent Assay

chapter Chapter 18|4 pages

Radioimmunoassay

chapter Chapter 19|6 pages

Polymerase Chain Reaction

chapter Chapter 20|10 pages

Biosensors/Glucometer

chapter Chapter 21|4 pages

Glycated Hemoglobin

chapter Chapter 22|8 pages

Enzymes

chapter Chapter 23|4 pages

DNA Isolation from Blood and Tissue

chapter Chapter 24|7 pages

Mass Spectrometry

chapter Chapter 25|10 pages

Flow Cytometry

chapter Chapter 26|5 pages

Centrifugation

chapter Chapter 27|8 pages

Automation

chapter Chapter 28|3 pages

Cerebrospinal Fluid Examination

chapter Chapter 29|4 pages

Oxalic Acid