ABSTRACT
Before start of the experiment, application was made to the Direcção de Serviços de Meios de Defesa da Saúde, Bem-estar e Alimentação Animal da Direcção Geral de Veterinararia (Portugal). The animal experimental procedures were performed in Estação Zootécnica Nacional (EZN)—Instituto de Tecnologia Biomédica-Santarém-Portugal, in accordance with the International Guiding Principles for Animal Research. The animals are aquired in Universidade de Córdova-Espanha, and maintained in the EZN during the entire experimental period were daily observed by the
medical staff of the EZN. Non carious and periodontal sound mature incisors and premolars from 3 male Beagle dog (age 5 months; body weight 11.73 ± 1.13 kg) were selected. All experimental procedures were performed with the animals under general anesthesia, accomplished by pre-anesthetic sedation with 0.05 mg/kg of body weight of acepromazine (Calmivet®- Vetoquinol-Lure-France) administered intramuscular and anthetic induction with 10 mg/kg of body weight administered intravenous of thiopental (Pentotal® -Queluz de Baixo-Portugal). Finally, intubation was made and maintenance of anesthesia was a mixture of oxygen and 1-2% of isoflurane (Isoflo, Veterinaria Esteve, Barcelona-Spain). Throughout the duration of general anesthesia, the dog’s received normal saline solution intravenously.
