Figure 2 Differential pulse anodic stripping voltammograms from an HSA immunoassay as illustrated in Figure 1 for a series of HSA standards of increasing concentration (A–F) in 0.1M citrate pH 1.5. Voltammetry conditions: hanging mercury drop electrode (volume 0.37 µL), deposition time 5 min, deposition potential −800 mV vs. Ag/AgCl scan rate 2 mV s,

doi:10.1201/9780203908907-151

Chapter

Figure 2 Differential pulse anodic stripping voltammograms from an HSA immunoassay as illustrated in Figure 1 for a series of HSA standards of increasing concentration (A–F) in 0.1M citrate pH 1.5. Voltammetry conditions: hanging mercury drop electrode (volume 0.37 µL), deposition time 5 min, deposition potential −800 mV vs. Ag/AgCl scan rate 2 mV s,

Chapter

ABSTRACT

Five types of amperometric detection have been applied for enzyme-based ECIA. They are flow-injection analysis with electrochemical detection (FIAEC), liquid chromatography with electrochemical detection (LCEC), amperometric detection with interdigitated array electrodes (IDA), rotating disk electrode (RDE) amperometry, and scanning electrochemical microscopy (SECM). Of these, the two conventional types, FIAEC and LCEC, shall be discussed in this section, leaving the discussion of the other types to Section V on miniaturized immunoassays.