ABSTRACT
Among several types of fluorophore, styryl dyes represent the most popular family of fast dyes, RH-421, di-4-ANEPPS, and di-8-ANEPPS being the most important members of this family. The spectroscopic properties of these dyes have been shown to change linearly with membrane potential changes in normal physiological range of transmembrane voltages.[1] The orientation of the molecules of these dyes in the cell membrane is ensured by the presence of lipophilic and hydrophilic groups at opposite ends of the molecule.
