ABSTRACT
Reaction conditions for the enzyme and thus the conversion, efficiency and work-up of the reaction may be suboptimal at this stage, but the main criteria is access to the desired compound with the correct stereochemistry and adequate purity. In the research stage, lengthy enzyme evolution campaigns possible due to the short timelines, but in development, such efforts can be undertaken and often yield an improved biocatalyst and synthetic route with fewer reaction steps and higher yields than classical chemical alternatives. Synthesis of pharmaceutical metabolites as analytical standards and for structure elucidation is a vital step for enabling pharmacokinetic studies during drug development process. Malaria is a disease caused by single-celled parasitic microorganisms of the Plasmodium group that are transferred to humans by a bite of an infected female anopheles mosquito. In parallel to the kinetic resolution approaches, an asymmetric synthesis for preparation of chiral amine S-4 was studied for improved cost and atom efficiency towards the production of Cipargamin.
