ABSTRACT
Enzymes are natural catalysts that facilitate the majority of the reactions occurring in biological systems. Metagenomics is an approach for accessing global microbial genetic diversity from environmental sources. The catalysis occurs at the lipid–water interface, a phenomenon, termed interfacial activation. Lipase-catalyzed reactions have received much attention in recent years, as they are increasingly being used in biotechnological applications in the chemical, food, and pharmaceutical industries. Biochemical studies clearly showed that the LipR1 protein is quite stable in most of the organic solvents and is also quite stable up to 50°C temperature. The initial reaction with lipase yielded the maximum conversion of 40% after 48 h of the reaction, and the conversion did not increase beyond this, even after 96 h of the reaction. A few studies have been reported on the synthesis of S-1-(1-naphthyl) ethanol by asymmetric reduction of prochiral ketone using microbes, asymmetric chemical catalysis, or lipase-catalyzed kinetic/dynamic resolution.
