ABSTRACT
Contents 3.1 Introduction ..................................................................................................................... 22 3.2 Proteome Analysis by 2DE ............................................................................................... 22
3.2.1 Sample Matrix Considerations .............................................................................. 22 3.2.1.1 Whole Larval Proteomes ......................................................................... 22 3.2.1.2 Muscle Proteomes ................................................................................... 24 3.2.1.3 e Degradome ...................................................................................... 24
3.2.2 Basic 2DE Methods Overview ...............................................................................25 3.2.2.1 Sample Extraction and Cleanup ..............................................................25 3.2.2.2 First-Dimension Electrophoresis ..............................................................25 3.2.2.3 Equilibration .......................................................................................... 27 3.2.2.4 Second-Dimension Electrophoresis ......................................................... 27 3.2.2.5 Staining .................................................................................................. 28 3.2.2.6 Analysis .................................................................................................. 28
3.2.3 Protein Identi cation by Peptide Mass Fingerprinting .......................................... 28 3.3 Applications of 2DE in Seafood Analysis ..........................................................................31
