ABSTRACT
An expressed sequence tag (EST) is a short complementary DNA (cDNA) sequence derived from messenger RNA (mRNA) (Adams et al. 1991). ESTs are produced by single read sequencing of cDNA clones from either the 3’- or 5’-end untranslated regions (UTR). ESTs provide direct evidence for all sampled transcripts and are very important resources for transcriptome studies (Nagaraj et al. 2007).
