ABSTRACT

In1961,Stemetal.(88)carriedoutthefirstexperimentusinganti-D.Theyinjected16 Rh-menwithABO-compatible,D+RBCs,coatedinvitrowithanti-Dsufficienttoproduce astronglypositivedirectantiglobulin(Coombs')reaction.Fourteenmenwereinjectedfive times;twowereinjectedfourtimes.Noneofthe16developedanti-D.When10ofthe16 weresubsequentlyinjectedwithD+RBCsnotcoatedwithanti-D,5(50%)becameD immunized.