ABSTRACT
ABSTRACT: Loop-Mediated Isothermal Amplification (LAMP) is a novel and rapid nucleic acid amplification method. We used this method to develop a simple and rapid way to quantify the Heat Shock Protein (HSP) genes of Sebastiscus marmoratus. After exposure to different concentrations of the water-soluble fraction of crude oil for 1, 5 and 10 days, the levels of HSP60, HSP70 and HSP90 mRNA in the liver of S. marmoratus were measured using Real-Time PCR (RT-PCR) and Real-Time LAMP (RT-LAMP). The change patterns of all three gene levels measured using RT-LAMP were the same as those measured using RT-PCR, indicating that the RT-LAMP is an effective method for monitoring HSP mRNA. The HSP mRNA levels in the liver of S. marmoratus were all inhibited after exposure, which is different from the results of various other studies concerning HSPs.
