ABSTRACT
Brown macroalgae are distributed on rocky shores of both the hemispheres. They play a key role in the coastal primary production, providing food and habitat for, that is, invertebrates and fish. Even though many physiological studies on brown algae were performed, molecular studies are still scarce. Molecular techniques, that is, gene expression profiling, greatly advances our comprehension of how organisms respond to abiotic and biotic stress. The rapid developments in molecular techniques during the recent years offer the possibility to generate cost-effective large-scale sequencing data from nonmodel organisms. Here, a rapid and effective method for extracting high-quality RNA is presented, which has been successfully applied for downstream applications, for example, qRT–PCR and Illumina RNA seq.
Keywords: Brown algae, nonmodel organisms, RNA extraction, RNA quality, transcriptomics, gene expression
