ABSTRACT
This chapter presents the results of three specific practical studies exploiting the applications of Surface plasmon resonance (SPR) technology for the investigation of processes in (bio)molecular systems involving the conformational changes in their constituents. The first study is focused on the real-time SPR characterization of the cooperative interactions between aggregate-forming bovine serum albumin and oppositely charged linear macromolecule poly(acrylic) acid, resulting in the estimation of the kinetic constants of conformational transformations for different pH values. The second study deals with the SPR transduction of the human olfactory receptor 17—40 stimulation with several odorants, revealing the possibility of developing a high-sensitive SPR-based bioelectronic nose. The third study reports on the characterization of conformational changes in acrylamidophenylboronic acid-acrylamide hydrogels upon interaction with glucose by SPR and complementary techniques, resulting in the development of a novel glucose-sensing approach.
