ABSTRACT
The affinity between the covalently bound nucleic acid component and the partner of the mobile phase to be isolated is highly stereospecific. The interaction does not only lead to a complex formation. The subsequent displacement of the molecule from the complex will depend on the three-dimensional orientation, on the group and position on the ligand molecule through which it is immobilized to the support, to some extent on the chemical method by which the column is made, and on the spacer distance between the ligand and the supporting matrix. A lot of literature has been concerned with designing simplified chemical methods for preparing polymer support derivatized with nucleic acid components. Linkage through the sugar hydroxyl groups is the easiest way of immobilizing nucleotides. These nucleoside gels possess very high concentrations of covalently bound nucleoside residues. Moreover, the gels may be used either in organic or aqueous medium and show good flow rates in column chromatography.
