CONTENTS

RNase-free conditions are very important. All the equipment must be sterilized at 180

°

C for 3 h, and water used for solutions must be sterile or DEPC-treated (

see

Appendix B1.2).

Sequences of interest are amplified within cells in the presence of:

• Specific short primers (synthetic oligonucleotides) which flank the target sequence

➫

See

Section 5.3.2.