ABSTRACT
S p e c ific ac tiv ity , a c o m m o n fo rm o f rep o rtin g d eg ra d a tio n ac tiv ity , is ta k en as m o le s (o r m ic ro m o les) o f su b stra te lo st p e r tim e p e r u n it m ass o f p ro te in . B io m ass (X ) can b e re la ted to p ro te in c o n c en tra tio n , so sp ec if ic ac tiv ity is d e sc r ib ed by
W h e n S is m u ch le ss th an K s , th e M o n o d eq u a tio n s im p lif ie s to
T h e a p p a re n t o v e ra ll ra te co n s tan t is g iv en by p max X / Y K s. T h is p e rm its th e p s e u d o f irs t-o rd e r ra te co n s ta n t (w h en S is sm a ll) to b e re la ted to th e sp ec if ic ac tiv ity b y th e fo llo w in g co n v e rsio n :
T h e w h o le ce ll s tu d y c o n d u c te d h e re re su lted in w h o le ce ll “ac tiv ity ” ran g in g fro m 0 .0 0 4 to 0 .0 2 p m o l 1 ,2 -D C A /m g p ro te in /m in . K eu n in g e t al. rep o rted sp e c if ic ac tiv ity o f a c ru d e ce ll ex tra c t a t 0 .1 6 p m o l/m g p ro te in /m in [21]. P u rif ied d eh a lo g e n ase ex tra c ts h av e sh o w n 6 p m o l/m g p ro te in /m in [301.
