ABSTRACT
The same rationale used to obtain the linear dimensions of an object based on the use of standard calibrated rules can be applied directly to the measurement of the dimensions of the minute specimens being observed with a compound light microscope. Length and width dimensions of microscopic-sized specimens can be obtained with an ocular micrometer that has been calibrated with a stage micrometer. Figure 4.4 shows the placement of a micrometer at the ocular’s intermediate image plane. Next, values are assigned to the ocular’s equally spaced, arbitrary divisions, by calibration with a standard stage micrometer.
