ABSTRACT
Ion-exchange chromatography (IEC) is a process that allows the separation of ions and polar molecules based on charge-charge interactions between the proteins in the sample. In biochemistry it is widely used to separate charged molecules. An important area of the application is extraction and puri”cation of biologically produced substances such as proteins and amino acids, for example, DNA and RNA. IEC can also be used for the separation of peptides and oligonucleotides.
