ABSTRACT

The development of suitable gene transfer systems is a major prerequisite for molecular genetic and biochemical investigations in various organisms, including the filamentous fungi. Molecular transformation involves two main components: (a) an appropriate vector containing a selectable marker and (b) a suitable transformation procedure for the introduction of the corresponding vector into the respective fungal system. Finally a sustainable replication of the transforming DNA has to be achieved either by integration of the vector into the genome or by applying an autonomously replicating vector system.