ABSTRACT
H.Schütte The example chosen is harvesting cells of Saccharomyces cerevisiae by crossflow filtration.
Principle and Aim of the Experiment Cross-flow filtration is an alternative method to the classical solid/liquid
separation techniques such as sedimentation, centrifugation, separation or filtration. Microfiltration membranes with a pore diameter between 0.2 µm and 0. 45 µm, either as planar membranes in a flat holder or folded or spiral membranes in the appropriate holders, are most often used and in some instances tubular membranes in the form of hollow fibres or capillaries. The method described here using micropore membranes is a means of obtaining cells from fermenter cultures. The concentration of biomass is in principle a cyclic process in which the biomass is concentrated to the extent required for the next step in the process.
