ABSTRACT
Enzymatic substrate regeneration is a tool to enhance the sensitivity of enzyme electrodes both for substrate analysis and immunoassays.
The combination of immunoreactions and electrode based substrate recycling connects very specific recognition of an analyte with highly sensitive detection. Most important for this field of application is the sensitivity, which permits the detection of a label at very low concentration. For enzyme immunoassays in particular, several goals have to be achieved:
iii product is indicated very sensitively, iii enzyme has high turn over number, iii enzyme substrate produce only a low background response, iv substrate and product are stable, iv close pH optimum of marker enzyme and detector.
