ABSTRACT
Another important conclusion is that until recently, most media formulations and modes of insect cell cultivation were devised empirically. But now the studies with Sf 9 cells with serum supplemented and serum free media as a vehicle to illustrate metabolic patterns and culture behaviour with process development implications have been demonstrated. This has led to obtain higher cell density and lengthy adaptation period for the cells. This has helped to overcome the long culture lags. In fact, with the current trend of High-Throughput Screening (HTS) in both the pharmaceutical and agrichemical industries, there is a great value placed on the ability to overexpress target sites of importance in cell lines. Sf 9/High Five and Schneider/Drosophila cells are very popular for the expression of mammalian receptors. Lately, a number of reports have emerged suggesting that these cells are now being used to express insecticidal target sites for screening (HTS).
