ABSTRACT
The endolysosomal system is a series of membrane-bound organelles which is required for maintaining physiological functions of the cell, including transport of cargo molecules from the extracellular environment into the intracellular space and vice versa. The protocols of voltage and current clamp experiments in endolysosomal patch clamp are designed as in conventional patch clamp. One of the main challenges in endolysosomal patch clamping is the production of micropipettes. Transient receptor potential mucolipins 1, 2 and 3 constitute a family of endolysosomal cation channels with homology to the transient receptor potential superfamily. Endolysosomal organelles are categorized into different subgroups including recycling endosomes, endosomes, late endosomes and lysosomes. The main drawback of both methods is that the new membrane environment is fundamentally different from the endolysosomal membranes. In particular, the specific membrane composition and local membrane proteins are very different.
