Pigment production in Ajuga cell cultures
This chapter provides clear-cut demonstrations of secondary metabolite accumulation in unorganized cultures from different genotypes of Ajuga. It illustrates the influence of chemical microenvironmental factors, specifically, carbon source, on the levels of pigmentation produced by Ajuga callus. The chapter demonstrates the influence of explant size and source on productivity and pigment expression in amorphous masses of callus. It then features a variegated chimeral Ajuga genotype, and illustrates the variation in productivity and performance of callus isolated from different sectors of this chimeral leaf. The chapter also examines the influence of physical microenvironmental treatments on anthocyanin production from cell cultures. Ajuga pyramidalis 'Metallica Crispa', and the variegated chimeral selection A. reptans 'Burgundy Glow' are both readily available at most garden centers as flats of stoloniferous, spreading plants ready to plant in the landscape. Secondary products, including anthocyanins and other pigments, are often preferentially produced at specific ranges of light wavelengths.