ABSTRACT
This chapter outlines low-angle laser light scattering photometer (LALLS) detector design and the principles of Size exclusion chromatography (SEC) /LALLS, with selected examples of its application in biopolymer analysis. Dilute-solution visible light scattering can be interpreted within the framework of fluctuation theory. This theory dictates that light is scattered because of changes in the polarizability of the solution, which, in turn, is caused by random fluctuations in the solute concentration of small volume elements. The KMX-6 can be configured for use in quasi-elastic light scattering experiments by attaching a photon counting interface to the PMT and feeding the output to a digital correlator. The CMX-100 Light Scattering Detector was designed specifically for SEC. The large volume of data generated by an automated multi-detector SEC system demands such data-handling capability for efficient data acquisition, storage, retrieval, and analysis. Laboratory data systems with such capabilities are available together with appropriate software.
