chapter  13
8 Pages

Mapping transcription factor binding sites using ChIP-chip – general considerations

ByRebecca Martone, Micheal Snyder

This approach was first invented for yeast (5, 6) and has now been successfully performed for a multitude of different transcription factors in many organisms (see Table 13.1). Several important considerations are critical to the ChIP-chip approach including experimental design, array selection, and data analysis. Here we will focus on experimental considerations and then report several studies employing ChIP-chip, which are meant to highlight the various microarray platforms suitable for this type of analysis.