chapter  20
Application of PCR in Plant Virology
ByRalf Georg Dietzgen
Pages 30

Eleven years ago, the journal Science named the polymerase chain reaction (PCR) a major scientific development and chose DNA polymerase as its "Molecule of the Year" (Guyer and Koshland, 1989). Since then, this extremely powerful and versatile technique has literally revolutionized research in molecular biology. In plant virology, PCR has been widely adopted and its use in an ever increasing number of novel applications appears to be limited only by the imagination of the researcher. The potential power and sensitivity of PCR is due to the exponential amplification of small amounts of specific target DNA sequences in vitro.