ABSTRACT

Nanotechnology driven biocatalytic techniques have emerged as a highly efficient, practically easy, appropriate and reproducible technique for the enzyme immobilisation. Researchers were successful in developing matrix based on carbon nanotubes (CNTs) for enzyme immobilization. The enzymes immobilised on CNTs can withstand high temperature that results in enhanced and stable performance of the enzyme. This chapter focuses on CNT-based enzyme immobilisation and its implications in biofuel. Catalyst-assisted chemical vapour deposition of hydrocarbons is the most popular method of CNT synthesis due to the high quantitative yield of the process and often this process is assisted by the presence of iron and cobalt catalysts. The use of plant materials such as coconut oil as substrates to synthesise multi-walled CNTs (MWCNTs) of 80–90 nm diameter served as a green strategy of nanoparticle synthesis following the principles of vapour deposition methods. Different types of biomolecules and polymers were used to functionalise CNTs.