ABSTRACT
Rachayya M. Devarumath, PhD; Sachin B. Kalwade, PhD; Pranali A. Kulkarni, PhD; Suman S. Sheelavanthmath, PhD; and Penna Suprasanna, PhD
Sugarcane belongs to the grass family (Poaceae) and is the source of about 75% of the world’s sucrose, with a value of more than US$150 billion per year. Apart from its traditional use as a source of sugar, sugarcane is fast becoming a source for ethanol and biomass production, which is an alternative energy source to the nonrenewable resources in many countries, helping to build up their economies. Sugarcane is cultivated in the tropical and
CONTENTS
7.1 Introduction ........................................................................................................................ 191 7.2 Sugarcane Initiatives ......................................................................................................... 195
7.2.1 Sugarcane Synteny and Colinearity with Other Poaceae Members ............... 195 7.2.2 Sugarcane Genome ................................................................................................ 196
7.2.2.1 Sequencing ............................................................................................... 197 7.2.2.2 Gene Discovery ....................................................................................... 199
7.3 Sugarcane Transcriptome .................................................................................................200 7.3.1 Sources of Transcriptomes ....................................................................................200 7.3.2 Functional Integrity of Transcriptomics in Sugarcane ..................................... 203 7.3.3 Comparative Transcriptomics and Reverse Genetics ....................................... 206 7.3.4 Transcriptome Polymorphism for Functional Studies ..................................... 207
7.4 Transcriptome Changes in Response to Abiotic and Biotic Stresses ......................... 208 7.5 Integration of Metabolomics with Genomics ................................................................ 212 7.6 Sugarcane Proteomics ....................................................................................................... 214 7.7 Sugarcane Molecular Breeding ........................................................................................ 216
7.7.1 DNA Markers in Sugarcane ................................................................................. 216 7.7.2 Genetic Linkage Maps in Sugarcane ................................................................... 217 7.7.3 QTLs in Sugarcane ................................................................................................. 219 7.7.4 Molecular Markers for Diagnostics ..................................................................... 221
7.8 Genetic Transformation in Sugarcane ............................................................................ 221 7.9 Metabolic Engineering of Sugarcane and Biofactory ...................................................227 7.10 Genetic Variability through In Vitro Culture and Mutagenesis ..................................228 7.11 Conclusions and Future Perspective ...............................................................................229 References .....................................................................................................................................230
