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more specific information of population composition can be obtained by secondary analysis of the DGGE/TGGE banding pattern. Individual bands (fragments) may be excised from the gel, subjected to a second round of PCR amplification and sequenced (MacNaughton et al. 1999). Alternatively, the DGGE/TGGE patterns can be transferred to nylon membranes and then challenged with appropriate oligonucleotide probes to identify specific populations within die microbial community (Muyzer 1993, Santegoeds et al. 1998, Stephen et al. 1998, Heuer et al. 1999). The
DOI link for more specific information of population composition can be obtained by secondary analysis of the DGGE/TGGE banding pattern. Individual bands (fragments) may be excised from the gel, subjected to a second round of PCR amplification and sequenced (MacNaughton et al. 1999). Alternatively, the DGGE/TGGE patterns can be transferred to nylon membranes and then challenged with appropriate oligonucleotide probes to identify specific populations within die microbial community (Muyzer 1993, Santegoeds et al. 1998, Stephen et al. 1998, Heuer et al. 1999). The
more specific information of population composition can be obtained by secondary analysis of the DGGE/TGGE banding pattern. Individual bands (fragments) may be excised from the gel, subjected to a second round of PCR amplification and sequenced (MacNaughton et al. 1999). Alternatively, the DGGE/TGGE patterns can be transferred to nylon membranes and then challenged with appropriate oligonucleotide probes to identify specific populations within die microbial community (Muyzer 1993, Santegoeds et al. 1998, Stephen et al. 1998, Heuer et al. 1999). The
ABSTRACT
Limitations of M olecular Methods
Extraction of Nucleic Acids