chapter  10
20 Pages

Detecting Optically Inactive Analyte: MC-CE Device Integrated with QDs/LIF Detection for Determination of Acrylamide in Food

ByQidan Chen, Ying Sing Fung

AA in Food ................................................................................... 173 10.2 CE Methodologies for Detecting Optically Inactive AA ..................... 174

10.2.1 Choice of Separation Mode ........................................................ 174 10.2.2 Operations and Procedures ....................................................... 175

10.3 Integrating QDs with LIF for Indirect Determination of AA ............. 176 10.3.1 Preparation of CdTe QDs and QDs-AA Complexes ............... 176 10.3.2 Characterization of Prepared QDs and QDs-AA Complex ...... 177 10.3.3 Study of QDs-AA Interaction .................................................... 178

10.4 MC-CE Device Integrated with QDs-LIF Detection for Indirect AA Determination ..................................................................................... 181 10.4.1 Design and Layout of the MC-CE Device Integrated with

QDs-LIF Detection ....................................................................... 181 10.4.2 Optimizing Operation Conditions of MC-CE/QDs-LIF

Device for Indirect Determination of AA ................................ 182 10.5 Application of the MC-CE/QDs-LIF Device for Indirect

Determination of AA in Potato Chips .................................................... 184 10.5.1 Sample Preparation and Cleanup Procedures ........................ 184

Determination of contaminants at trace levels in food has become a hot topic for analytical method development due to the increasing demand to cover various analytes at trace levels which are often beyond the detection limits of conventional methods.