chapter  5
Production of siRNAs with the Application of Deoxyribozymes
WithGraeme Doran, Muhammad Sohail
Pages 14

Exogenous application of siRNAs is becoming a widely used approach to achieve gene knockdown.1 However, siRNAs targeted to different regions of a gene exhibit different efficacies,2-4 and often several reagents may have to be screened for optimal activity. Chemical synthesis of RNA remains expensive, making this approach uneconomical. Therefore, this is highly desirable to develop alternative methods of producing siRNAs.