chapter  11
RNAi in Caenorhabditis Elegans
WithFemke Simmer and Ronald H.A. Plasterk
Pages 16

Microscopy, and Genetic Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178 Protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178 Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 180

The nematode Caenorhabditis elegans is a useful animal model organism to study complex biological phenomena (Figure 11.1).1 Most C. elegans genes have homologues in other organisms, including humans, and many biological mechanisms are well conserved. Importantly, C. elegans is easily maintained in the laboratory and is amenable to a large range of experimental methods. Experiments in C. elegans have, for instance,

played an important role in elucidating the RAS-signaling pathway and aspects of apoptosis and in the discovery of RNA interference (RNAi).