chapter  12
Delivery of RNAi Reagents in C. elegans by Microinjection
WithPeter J. Appleford, Alison Woollard
Pages 18

Caenorhabditis elegans was the first metazoan for which the entire genome sequence was made available.1 This, coupled with the ease of maintenance, favorable genetic properties, and invariant cell lineage, makes C. elegans an excellent model organism for biological analysis. With the sequence of all 19,000 or so genes available, it is natural that research groups should seek to use a reverse genetic approach in order to knock out genes of interest. As well as being of great relevance to established worm labs, reverse genetic analysis of C. elegans genes has also become an increasingly popular approach in nontraditional worm research environments.