chapter  3
Oligonucleotide Scanning Arrays in the Design of Small Interfering RNAs
WithValentine M. Macaulay, Muhammad Sohail
Pages 22

Murine Tumor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 3.3.3 Correlation of IGF1R Gene Silencing with Biological Efficacy . . . . 47

3.4 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Small interfering RNAs are powerful gene silencing tools. The most potent RNAi effectors in Drosophila are RNA duplexes with 19 bp of homology to the target gene and 2 nucleotide 3' overhangs.1,2 Nearly two years ago these 21 to 23 nucleotide small interfering RNAs (siRNAs) were shown to effect potent and sequence-specific silencing of exogenous and endogenous genes in mammalian cells.1