chapter  2
Use of the λ Red-Recombineering Method for Genetic Engineering
ByZhanmin Fan, Ling Yuan and Ranjini Chatterjee Joanna I. Katashkina, Yoshihiko Hara, Lyubov I. Golubeva,
Pages 21

Background Pantoea ananatis belongs to the Enterobacteriacea family. e P. ananatis strain AJ13355 (SC17) was isolated from soil in Iwata-shi (Shizuoka, Japan) as a bacterium able to grow at acidic pH and showing resistance to high concentrations of glutamic acid [1]. ese physiological features made this organism an interesting object for biotechnological studies, and for this reason its genome has been sequenced by Ajinomoto Co. (unpublished results). Nevertheless, up to the recent past, the absence of ecient genetic tools has hampered manipulations of this bacterium and retarded both basic research and applied investigations.