ABSTRACT
DNA assembly is the workhorse of molecular biology. Most molecular biology projects begin with joining some combination of DNA pieces together in the service of some larger goal such as testing a biological hypothesis. For 20 years or so, DNA assembly was largely limited to techniques involving in vitro restriction digest and ligation.1 While effective, these techniques often suffered from several limitations. One limitation was the existence of sequence constraints in the nal products, as well as construction intermediates,
CONTENTS
2.1 Introduction ..........................................................................................................................35 2.2 Theory and Considerations in Double-Stranded DNA Assembly ...............................36 2.3 Assembly Methods in Detail ..............................................................................................38
