ABSTRACT

As the ambition of synthetic biologists grows, systems and synthetic approaches to biology are beginning to be used concomitantly. Nowhere is this more apparent than in metabolic engineering applications where synthetic biologists have staked much on the promise of synthetic biology to build cellular factories producing cheap abundant biofuels and diverse natural products. To achieve these challenging goals, it is necessary to standardise metabolic network engineering so that product yields can be maximised against the natural tolerances of host organisms for non-native genes and biochemical pathways. Such standardisation requires gene measurement to be tuned and characterised and in this chapter current

CONTENTS

3.1 Introduction .......................................................................................................................... 67 3.2 Standardisation in Conventional Biology ........................................................................68