chapter  14
22 Pages

Molecularly Imprinted Polymer-Based Biosensors

ByMihrican Muti, Arzum Erdem

Molecular imprinting is a versatile technique for the preparation of synthetic receptors, on the basis of complexes between a template molecule and polymerizable monomers. After polymerization process, the products are called molecularly imprinted polymers (MIPs). These polymers are prepared by using cross-linked polymers containing cavities specic to an analyte. These cavities are created by copolymerization of cross-linking monomers and functional monomers along with an imprinting molecule or template. In the molecular imprinting technique, the rst important step is the interactions of template molecule with functional monomer before polymerization. The monomer-template interactions may occur in two different ways: covalent or noncovalent interactions. The second important step is to remove the template molecule from the resulting polymer to generate the vacant recognition sites for the template molecule in terms of both functional groups and size [1-3]. The MIP then selectively rebinds to the analyte compound [4,5]. By this way, MIP technologies

CONTENTS

14.1 Introduction ................................................................................................ 373 14.1.1 Synthesis of Molecularly Imprinted Polymers .......................... 374

14.1.1.1 Synthesis Protocols ......................................................... 378 14.2 MIP-Based Biosensor Developed for Monitoring of Food

Toxicants and Environmental Pollutants ............................................... 381 14.2.1 MIP-Based Biosensor for the Detection of Food Toxicants ...... 381 14.2.2 MIP-Based Biosensor for the Detection of Environmental

Pollutants .........................................................................................385 14.3 Conclusion .................................................................................................. 389 Acknowledgments .............................................................................................. 390 References ............................................................................................................. 390

imitate biological antibody systems that function on the basis of specic binding sites where an antigen binds strongly to an antibody [5].