chapter  22
34 Pages

Oligonucleotide and DNA Microarrays as Versatile Tools for Rapid Diagnostics

BySusana Campuzano, María Pedrero, José M. Pingarrón

A DNA array is an orderly arrangement of samples where matching of known and unknown DNA chains is done based on base-pairing rules. In fact, DNA microarrays work on the principle of base pairing that allows probes to hybridize targets on the microarray. Traditional array experiments make use of common assay systems such as microplates or standard blotting membranes. The sample spot sizes are typically lower than 200 μm in diameter and a microplate usually contains thousands of spots.