A Strategy for Rapid Identification of Novel Therapeutic Leads from Natural Products
CONTENTS 14.1 Introduction 14.2 Initial Fractionation of Microbial Crude Extract by Countercurrent Chromatography
14.3 Liquid Chromatography-Mass Spectrometry 14.4 Strategy for Dereplication 14.5 Rapid Identification of Known Compounds 14.6 Identification of Novel Leads by High-Speed Countercurrent
Chromatography Fractionation 14.7 Lead Compounds 14.8 Experimental Section
14.8.1 Sample Preparation 14.8.2 Countercurrent Chromatography Fractionation 14.8.3 Liquid Chromatography-Mass Spectrometry Analysis
The discovery of novel, small molecules through screening secondary microbial metabolites is still an important and fruitful activity in pharmaceutical and biotech industries. However, the isolation and structure elucidation of lead compounds is often a tedious and time-consuming process especially when the compounds being sought may only be present in infinitesimal quantities. When one considers, for example, that microorganism extracts have thousands of constituents, the difficulties in separating out one particular component can be appreciated.