chapter  7
10 Pages

Development of New Label Enzymes for Bioluminescent Enzyme Immunoassay

The use of these techniques has been facilitated by the expression of various luciferase and phosphoprotein genes in

Escherichia coli

or

Saccharomyces cerevisiae

.

Recombinant firefly luciferase is commercially available and has been used for enzyme immunoassay (EIA). However, firefly luciferase loses much of its activity when treated with the chemical cross-linking reagents used in production of conjugates. To address this problem, we have established two highly sensitive bioluminescent EIAs (BLEIAs) in which thermostable acetate kinase (AK) is used to label the conjugate.