chapter  10
18 Pages

Chemiluminescent Technology: Optimization of Western Blots and ELISAs Utilizing a Horseradish Peroxidase Label in Automated Systems

Western blotting and ELISAs are two of the most common techniques used by researchers today to analyze protein samples for various applications. Traditionally, color-producing substrates and radioactivity were used to analyze results in both these techniques. With the continued concerns about radioactive waste and contamination, along with the insensitivity provided by colorimetric substrates, enhanced chemiluminescent methods have become a viable and often preferable alternative. The use of luminol-based systems for the detection of the horseradish peroxidase (HRP) label has become very popular. Several enhanced chemiluminescent substrates are commercially available that utilize a phenolic compound as the enhancer.