chapter  11
10 Pages

A Luminol/Iodophenol Chemiluminescent Detection System for Western Immunoblots

Acknowledgment............................................................................................................................186 References ......................................................................................................................................186

Chemiluminescent (CL)-based procedures have recently become one of the most popular methods for the immunoblot detection of proteins and nucleic acids. CL has been explored as an detection method alternative to the use of radioisotopes because it eliminates the health hazard, cost, and disposal problems inherent in handling radioactive reagents. Furthermore, CL techniques are remarkably sensitive, require only short film exposure times, and produce sharply defined bands on immunoblots. At present, several chemiluminescent detection systems based on the use horseradish peroxidase (HRP),