chapter  21
18 Pages

Avoiding the Jaws of Using Lucigenin as a Chemiluminigenic Probe for Biological Superoxide: Determining When It’s Safe to Go Back into the Water

Biologically Derived Superoxide........................................................................................292 21.4 Lucigenin-Derived CL as a Probe for Detecting Intramitochondrial Superoxide.............293 21.5 Applications of Mitochondrial-Dependent Lucigenin-Derived CL

in Cell Biology and Toxicology .........................................................................................296 21.5.1 Assessment of Mitochondrial Maturation during Cell Differentiation ...............297 21.5.2 Assessment of Altered Mitochondrial Gene Expression .....................................297 21.5.3 Assessment of METC-Mediated Chemical Redox Cycling ................................297 21.5.4 Assessment of Mitochondrial Function during Aging.........................................298 21.5.5 Assessment of Mitochondrial Function in Disease States...................................299

21.6 Summary: The Margin of Safety and Lucigenin ...............................................................299 Acknowledgments ..........................................................................................................................300 References ......................................................................................................................................301

One of the products of cellular metabolism is the generation of the reactive oxygen species (ROS) superoxide and hydrogen peroxide.