chapter  42
18 Pages

BRET2: Efficient Energy Transfer from Renilla Luciferase to GFP2 to Measure Protein–Protein Interactions and Intracellular Signaling Events in Live Cells

Spectral Properties .................................................................................................541 42.4 Considerations for Detection of BRET

Assays ................................................................541 42.5 Plate Reader Design and Configuration.............................................................................542 42.6 Selecting the Optimal Configuration of the Fusion Universal Microplate Reader...........544 42.7 The BRET

Assay Technology...........................................................................................545 42.8 Applications of BRET

.......................................................................................................545 42.8.1 Study of G Protein-Coupled Receptor Activation in Intact Cells ......................546